Prolongation of seed viability and grain quality in rice by editing OsLOX1 using CRISPR/Cas9

Research Square (Research Square)(2022)

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摘要
Abstract Storage tolerance is an important agronomic character in rice (Oryza sativa L.). Lipoxygenase (LOX) is a key enzyme in lipid metabolism, and its activity directly affects the rate of aging in rice. Here, lipoxygenase gene OsLOX1 was edited by CRISPR/Cas9 in Ningjing 4. We found that OsLOX1 knock-out can delay seed vigor loss, lipid metabolism and quality deterioration after aging. In addition, combining the transcriptome analysis, we could conclude that OsLOX1 affected the transcription of multiple genes during storage, and was involved in seed storage tolerance by regulating lipid metabolism and antioxidant pathways such as phosphatase and acetaldehyde dehydrogenase. By knocking out LOX1 gene, a new rice material with high vigor and no exogenous vector skeleton was bred to improve the storage tolerance of seeds and provide material support for rice breeding in China.
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关键词
crispr/cas9,rice,grain quality,seed viability
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