A primer to directed evolution: current methodologies and future directions

Lara Sellés Vidal,Mark Isalan,John T. Heap,Rodrigo Ledesma-Amaro

RSC Chemical Biology（2023）

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摘要

This review summarises the methods available for directed evolution, including mutagenesis and variant selection techniques. The advantages and disadvantages of each technique are presented, and future challenges in the field are discussed.

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