Inoculation and Passaging of Adherent and Suspension Cells

Cell culture is an essential and necessary technique used in cell culture, unbiased with the growth of various organisms by introducing of various upcoming advancements in the field. The chapter describes the various possibilities and methods to inoculate adherent and suspension cell cultures to consistent development and maintenance of cell cultures to overcome biomedical problems. The chapter has also included the subculturing of monolayer cultures, media composition, cell detachment techniques, cellular synchronisation and the usage of antibiotics for controlling contamination in the culture.