Combinations of Probiotic Bacteria (Lactobacillus helveticus, Bifidobacterium longum, Lactobacillus rhamnosus, Sacchammyces boulardii) Restores the Skin Microbiome in Atopic Dermatitis

Combinations of probiotic bacteria may improve the quality of life and the effectiveness of standard treatment for atopic dermatitis. An open-label, placebo-controlled, randomized, pilot study in 2 parallel groups was conducted. The research group included 10 patients who received a combination of probiotic bacteria (Lactobacillus helveticus, Bifidobacterium longum, Lactobacillus rhamnosus, Sacchammyces boulardii) and 0.1% mometasone furoate cream for 28 days. The placebo group included 10 patients who received only 0.1% mometasone furoate cream. The study material was collected by the method of deep skin scraping. Identification of pure cultures of microorganisms was done using biochemical test systems. The combination probiotic led to a decrease in the number of gram-positive bacteria S. aureus from 5x106 to 1x102 CFU and E. faecalis from 5x106 to 3x103 CFU, as well as gram-negative bacteria Escherichia coli from 1x1011 to 1x105 CFU. In the probiotic group, at the end of the treatment, complete elimination of opportunistic gram-negative bacteria Acinetobacter lwoffii and microscopic fungi of the genus Candida were observed. On the 28th day of treatment, there was significant decrease in the SCORAD (16.5±4.1 vs 21.7±5.8, p=0.03) and DLQI index (6.6±2.2 vs 10.3±3.7, p=0.02) in the patients of probiotic group compared to the placebo group. The use of probiotic bacteria (Lactobacillus helveticus, Bifidobacterium longum, Lactobacillus rhamnosus, Sacchammyces boulardii) helped to restore the natural healthy microbiota of the skin, improving the effectiveness of treatment in atopic dermatitis patients.