Vector Tropism

Recombinant viruses expand the neurobiology toolbox and offer researchers an assortment of gene delivery options. Each type of virus varies in its capabilities and limitations, and therefore, there are important considerations in choosing the best viral vector system for each application. The suitability of a viral vector for gene delivery is dictated by factors such as genomic cargo capacity, duration and regulation of its transgene expression, immunogenicity, toxicity, and viral tropism. A myriad of permissive host factors is necessary to successfully express the viral genome and deliver transgenes. Viral tropism depends on the interaction of host and virion surface proteins for attachment and a concerted virus and host gene expression and assembly system. In this chapter, we hope to provide readers with a practical guide for selecting vectors for gene delivery in neuroscience applications.