Isolation of mouse embryonic fibroblasts (MEFs) from mouse embryos at E12.5 v1

Mouse embryo fibroblasts (MEFs) isolated from genetically altered mouse strains are a powerful resource for investigating the consequences of defined genetic mutations at the cellular level. Here we describe a protocol for the isolation of MEFs from mouse embryos collected at E12.5. MEFs obtained by this procedure are suitable for use in biochemical assays and for further genetic manipulation (e.g. viral transduction). This protocol was adapted from: Durkin ME, Qian X, Popescu NC, Lowy DR. Isolation of Mouse Embryo Fibroblasts. Bio Protoc. 2013 Sep 20;3(18):e908. doi: 10.21769/bioprotoc.908. PMID: 27376106; PMCID: PMC4928858.