Front Cover: Harnessing Split‐Inteins as a Tool for the Selective Modification of Surface Receptors in Live Cells (ChemBioChem 3/2023)

Split-inteins are master puppeteers of proteins, carrying out protein splicing in a traceless, auto-processing, site-specific manner that is biorthogonal to mammalian systems. The David group have utilized split-inteins to selectively modify cell-surface proteins. Here they show the utility of this method by labeling extracellular portion of epidermal growth factor receptor (EGFR) with a synthetic fluorophore for live cell imaging. The Front Cover graphic was designed by Eliot Fuks. More information can be found in the Research Article by Y. David et al.