Complete genome sequence of a novel caulimovirus isolated from Dregea volubilis

Abstract The complete genome sequence of a putative novel caulimovirus, tentatively named “Dregea volubilis caulimovirus 1.” (DVCaV1, GenBank accession no. MZ813180), infecting Dregea volubilis in China was determined using high-throughput sequencing (HTS). The complete open circular genomic DNA of DVCaV1 consists of 8,160 nucleotides (nt) and contains seven ORFs that encode a movement protein, an aphid transmission factor, a DNA-binding protein, a coat protein, a polymerase polyprotein, a translational transactivator protein and hypothetical protein, as well as two intergenic regions. This genome structure is typical of viruses of the genus Caulimovirus. Sequence comparisons analyses of the complete genome suggest that DVCaV1 is closely related to strawberry vein banding virus (NC_001725), and shares 43.3-50.3% nt sequence identity with caulimoviruses. Phylogenetic analysis showed that DVCaV1 clustered with viruses of the genus Caulimovirus,with strawberry vein banding virus being the closely related species. These results suggest that DVCaV1 is a new member of the genus Caulimovirusof the family Caulimoviridae. This is the first report of a caulimovirus infecting Dregea volubilis.