Comparative Assessment of Serum Biochemical Markers from Cattle and Buffaloes Vaccinated with Three Different Oil Adjuvant Vaccines of Hemorrhagic Septicemia from Punjab, Pakistan

Haemorrahgic Septiceamia (H.S) is a deadly, sub-acute livestock disease of cattle and buffalo in Southeast Asia including Pakistan. A whopping 98% case fatality rate renders vaccination a mandatory prophylactic control measure to counter HS. Very little information is available on serum biochemical profile of the HS vaccinated animals. This study was conducted to investigate the serum biochemistry of the vaccinated cattle and buffalo calves and adults alike. Three different experimental oil adjuvant vaccines were produced for Pasturella multocida serotype B:2 (locally prevalent). N=80 healthy cattle and buffalo (calves and adults) were divided into four groups. Each group consisted of 20 animals (10 calves and 10 adults). Group A was injected with vaccine prepared with Montanide ISA-50; Group B with vaccine prepared from Montanide ISA-206 while Group C with a vaccine produced from Liquid paraffin and Lanolin. Group D animals served as non-immunized control. Blood samples were collected from each animal at zero-day, after booster at 90 days, and then every quarter for one year. Twelve biochemical parameters were assessed by ANOVA and Kruskal Wallis Test. Post hoc multiple comparisons were done for glucose levels. It was concluded that vaccinating both cattle and buffalo calves and adults with H.S. vaccine formulated with three different adjuvants did not have a statistically significant effect on serum biochemistry of vaccinated animals compared to controls.