The ATP1A1 Gene Polymorphisms in Indonesian Beef Cattle

Abstract In this research, the direct sequencing method was used to identify the polymorphism and genetic diversity of the ATP1A1 gene in Indonesian beef cattle. The study used five breeds of local beef cattle with a total of 60 DNA samples (POGASI = 20, Jabres = 10, Bali = 10, Galekan = 10, and Madura = 10). The amplification of the ATP1A1 gene was using one set of primers (F = 5′- AGG GGT AGC CAG AGT TCC TA – 3′ and R = 5′ – CCC AAA GGT CAC GTG CTT TT – 3′). The result showed 6 SNPs in the APT1A1 gene, namely SNPs g.17293G/A, g.17356C/T, g.17359G/A, g.17541A/G, g.17585 A/G, and g. 17682C/T. Three SNPs were located in coding sequence nine, and the other SNPs were in intron 9 of the ATP1A1 gene. Based on the total population, the Chi-square test indicated that only two polymorphic loci (.17585 A/G and g. 17682C/T) fitted Hardy-Weinberg equilibrium (χ2<5.99). In conclusion, the polymorphic loci of the ATP1A1 gene can be used for genetic diversity and further association study to anti-heat stress traits of Indonesian beef cattle.