The antioxidant and anti-ageing activity of lyophilisate kersen (Muntingia calabura L) fruit in vitro

Kersen fruit (Muntingia calabura L.) is a wild plant used as food and used in medicine. This study aimed to determine the bioactivity of Kersen fruit as a candidate for active ingredients in cosmetics. Preparing the lyophilisate of kersen fruit was carried out through the lyophilization method using a freeze dryer. The lyophilisate of the Kersen fruit was then analyzed for its antiradical activity using the DPPH, ABTS radical scavenger, and FRAP reduction methods. The anti-ageing activity was evaluated using Elastase and Collagenase inhibition and fibroblast cell proliferation by the MTT method. The result showed that the lyophilisate of kersen fruit can inhibit DPPH, ABTS radical, and ferric reduction assay with strong activity. Lyophilisate of kersen fruit can interfere with Elastase and Collagenase activity with IC50 of 21.67 and 180.61 µg/mL, respectively. The results of the fibroblast cell proliferation activity showed that the lyophilisate sample could protect and increase the viability of fibroblast cells after exposure to H2O2 (IC90 value of 773.24 µg/mL). Based on these results, it can be concluded that Kersen fruit can be developed as an active ingredient in anti-ageing cosmetics.