Advancing spinal cord injury research with optical clearing,light sheet microscopy,and artificial intelligence-based image analysis

From the days of Ramon y Cajal's first sketches,neuroscientists have recognized the importance of visualizing the complex architecture of the central nervous system.In the past century,we have come to appreciate how the rich structural and functional complementarity of axons and cell types in the spinal cord make it uniquely suited for information transfer between the periphery and the brain.However,appreciating these relationships has been limited by the low-throughput histology techniques in common usage.