灵芝子实体多糖发酵工艺条件优化及抗氧化活性

Food Research and Development(2023)

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Abstract
为更好辅助和研究灵芝多糖,并进一步提高灵芝孢子亚门实体细胞壁基质(灵芝子实体)中灵芝多糖的抗氧化活性,选取产朊假丝酵母(Candida utilis)和扣囊复膜孢酵母(Saccharomycopsis fibuligera)作为灵芝混合多糖发酵菌种.以灵芝子实体多糖中的DPPH自由基清除率为响应值,结合单因素试验,并通过Box-Behnken设计的响应面法来进一步优化分析灵芝子实体多糖的发酵工艺.结果表明,最优的液态发酵反应条件:发酵时间60h、接种菌量3%、pH4、发酵温度26℃.灵芝子实体多糖在此最佳发酵工艺参数下,DPPH自由基清除率为90%.说明了灵芝子实体多糖经过发酵加工后,具有较强的天然抗氧化活性.
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Key words
Ganoderma lucidum fruit body,polysaccharides,process optimization,antioxidant activity,yeast fermentation
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