The evolving microenvironment of the human hepatocyte: Healthy vs. cirrhotic liver vs. isolated cells

•Healthy liver is characterized by low oxidative stress phenotype, robust metabolism and limited matrix turnover.•PHHs from healthy donors exhibit a stress phenotype, upregulate matrix turnover and downregulate metabolic function.•Ex vivo metabolic profile of PHHs may limit their capacity to serve as in vitro metabolism and toxicology models.