A structural and phase influences the physico-chemical, in vitro bioactivity of alumina-zirconia nanoparticles loaded chitosan-sodium alginate scaffold for biomedical applications

Presently, alumina-zirconia nanoparticles are prominently used in biomedical applications because of their valuable properties and augmented biological interactions. Amorphous (AZA) and tetragonal phase (AZT) nanoparticles are synthesized by spray pyrolysis. Three concentrations (10/15/20 mg) of synthesized alumina-zirconia nanoparticles were mixed separately with 4 wt% of chitosan-sodium alginate composite and developed as scaffolds using solvent casting. The physico-chemical and in vitro biological properties of the prepared samples are evaluated using appropriate techniques. The physico-chemical characteristics of AZT (tetragonal phase) nanoparticles and their scaffolds enhances in vitro biocompatibility, improve antibacterial activity against S. aureus and E. coli and also possess better in vitro cytotoxicity against human osteosarcoma (MG-63) cells compare than amorphous nanoparticles (AZA) and their scaffolds. From the analysis, AZT nanoparticles and AZT scaffolds are fit for biomedical applications, including tissue engineering and nano theranostics.