Bimetallic Metal-Organic Framework Nanoparticles for Monitoring Metabolic Changes in Cardiovascular Disorders

A group of disorders known as cardiovascular diseases (CVDs) affect the blood vessels that supply the heart muscle. CVDs are the leading global cause of death; however, the absence of a successful metabolomic strategy has impeded the advancement of CVD research. Because of this great challenge, bimetallic metal-organic framework (MOF) nanoparticles (NPs) have been developed for metabolic finger printings (MFs) in the study of molecular changes in CVD and coronary artery disease (CAD). A simple method for the design of various bimetallic MOF-NPs based on Zeolitic Imidazolate Frameworks (ZIFs) and bimetallic structure is reported. The serum MF was determined by examining 500 nL of natural serum in a few of seconds using the best bimetallic MOFs candidate (containing both Zn and Cu) integrated with assisted laser desorption/ionization mass spectrometry (LDI MS). Through the disclosed technology and a serum-based organization model, CVD patients could be differentiated from controls and CAD that have a sensitivity of 96% and a specificity of 93%. Furthermore, a portion of serum metabolic profiles with consistent changes can be used to track the progression of CVD, CAD, and controls. This study presents a state-of-the-art molecular approach for the metabolomic characterization of CVD and CAD that could one day be utilized to mimic clinical decisions in personalized therapy for cardiovascular disorders.