Supplementary Data from The Promoter-Associated Noncoding RNA <i>pncCCND1_B</i> Assembles a Protein–RNA Complex to Regulate Cyclin D1 Transcription in Ewing Sarcoma

Supplementary Experimental Procedures: Patient Datasets; Computational tools; SDS-PAGE and Western blot analyses; RTâ^'PCR analysis and Real-time quantitative PCR analyses; Immunoprecipitation; Chromatin immunoprecipitation; In vivo crosslinking and immunoprecipitation (CLIP); Sub-cellular Fractionation; Clonogenic Assay and Immunofluorescence analysis. Supplementary Figure Legends Supplementary Figures (1-8): SF1: Statistical analysis of CCND1 expression among Sarcoma samples; SF2: EWS-FLI1 regulates CCND1 expression; SF3: pncCCND1_B expression in 24 Ewing sarcoma patients;SF4: Identification of pncCCND1_B sequence; SF5: RT-qPCR analysis of CCND1 expression in Ewing sarcoma cells displaying different chromosomal translocation; SF6: Sam68 negatively affects CCND1 expression; SF7: YK-4-279 inhibits the interaction between EWS-FLI1 and DHX9, and trigger the formation of DHX9-Sam68 interaction; SF8: IGF-1 promotes Sam68 phosphorylation thus affecting its binding to pncCCND1_B RNA. Supplementary Table 1: List of oligonucleotides.