Supplemental Figures 1 to 7 from Cancer Stem Cells Regulate Cancer-Associated Fibroblasts via Activation of Hedgehog Signaling in Mammary Gland Tumors

Supplementary Figure 1. CAFs are generated in the stroma of Wnt-Met mammary gland tumors Supplementary Figure 2. CD140b+CD90+ cells isolated from Wnt-Met mammary gland tumors exhibit CAF features. Supplementary Figure 3. CAFs promote mammosphere formation and enhance the capacity of CSCs to form invasive 3D structures in Wnt-Met mammary gland tumors Supplementary Figure 4. CAFs from Wnt-Met mammary gland tumors display activation of Hedgehog signaling Supplementary Figure 5. Hedgehog signaling regulates CAFs in Wnt-Met mammary gland tumors Supplementary Figure 6. Therapeutic treatment of Wnt-Met mice with a Hedgehog pathway inhibitor depletes tumor stroma and prevents CSC expansion Supplementary Figure 7. Hedgehog signaling regulates the production of CAF ligands involved in the regulation of CSCs in Wnt-Met mammary gland tumors