Supplementary Figures 1-3 from Constitutive NOTCH3 Signaling Promotes the Growth of Basal Breast Cancers

Supplementary Figures 1-3 Supplementary Figure 1. NOTCH3 schematic (not to scale) showing residues for the furin (S1), metalloprotease (S2) and gamma-secretase (S3) cleavages. HD, heterodimerization; TM, transmembrane. Human S3 cleavage regions for NOTCH1 (P46531), NOTCH2 (Q04721), and NOTCH3 (Q9UM47) are aligned with the antigen sequence used to generate anti-V1662. Supplementary Figure 2. NOTCH3, but not other receptors or JAG1, modulates basal cell growth in soft agar. As in Fig. 4 for (A) MDA-MB-468 or (B) HCC70 cells. Supplementary Figure 3. NOTCH3 agonism stimulates tumor growth, whereas NOTCH3 antagonism slows tumor growth in vivo. As in Fig. 5B using HCC70 cells.