Supplementary Figure 5 from TMEM16A Induces MAPK and Contributes Directly to Tumorigenesis and Cancer Progression

PDF file - 69K, TMEM16A knock-down alters progression through cell cycle. T24 cells were transfected with TMEM16A siRNA or non-targeting control. These cells were then subjected to cell cycle analysis using FACS with propidium iodide staining. Transient knock-down of TMEM16A by siRNA blocks cell cycle progression from G1 to S phase (E; mean � SEM; n=3, **P<0.01, ***P<0.001). Caspase 3/7 activation was measured as a surrogate for apoptosis in T24 control and TMEM16A overexpressing cells. TMEM16A overexpression led to a reduction in caspase3/7 activity (B). Control and TMEM16A overexpressing cells were treated with AZD6244 (a specific ERK1/2 inhibitor), leading to an abrogation of TMEM16A-induced cell growth (C). Genetic knock-down of ERK1/2 with siRNA also abolished TMEM16A-induced growth (D).