Supplementary Materials and Methods, Supplementary Figures 1 through 9, and Supplementary Tables 1 through 4 from Early Epigenetic Downregulation of microRNA-192 Expression Promotes Pancreatic Cancer Progression

Supplementary Materials and Methods, Supplementary Figure 1. Representation of the DNA sequence upstream of the miR-192 gene and location of differentially methylated CpG dimers. Supplementary Figure 2. Assessment of in vitro DNA methylation of the CpG island sequence upstream the miR-192 transcription start site (TSS) by agarose gel electrophoresis after digesting with restriction endonuclease AciI. Supplementary Figure 3. Heatmap showing differentially expressed microRNAs in PDAC compared to normal pancreas tissue. Supplementary Figure 4. Heatmap displaying differentially expressed microRNAs in CP compared to normal pancreas tissue. Supplementary Figure 5. Heatmap showing a three-way comparison of microRNA expression in PDAC, CP and healthy pancreas tissues. Supplementary Figure 6. Result of bisulfite sequencing of the CpGI located 2.3 kb upstream of miR-181c. Supplementary Figure 7. Analysis of the effect of 5-azacytidine treatment on miR-192 expression in cell lines BXPC-3 and PANC-1. Supplementary Figure 8. Receiver Operating Characteristic (ROC) curve displaying the ability of miR-192 expression to discriminate between CP and PDAC samples. Supplementary Figure 9. Correlation of miR-192 expression and tumor stage. Supplementary Table 1. List of analyzed patient samples with information about relevant clinical annotations and the types of analysis that were performed with each sample. Supplementary Table 2. List of all DNA primer sequences used in this study. Supplementary Table 3. Summary of the methylation status of the analyzed CpG islands found upstream of the TSSs of the set of 13 miRNAs that exhibited differential expression of a similar nature in both CP and PDAC and had CpGIs within 10 kb upstream their TSSs. Supplementary Table 4. List of predicted miR-192 targets.