Supplementary Data from Targeting the Chromosomal Passenger Complex Subunit INCENP Induces Polyploidization, Apoptosis, and Senescence in Neuroblastoma

Supplementary Figures with legends- Supplementary Figures S1 through S7. (1) Fig.S1 shows the data about transcriptional regulation of INCENP by MYCN in NB cells and the results of Kaplan-Meier survival probability analysis based on the expression of INCENP in NB tumors in the Kocak cohort. (2) Fig.S2 shows the results of rescue experiment of INCENP knockdown by overexpressing a siRNA resistant form of INCENP and the growth inhibition effects in additional NB cell lines (KCNR and Kelly cells) upon INCENP depletion. (3) Fig.S3 shows the results of colony formation assay and soft agar assay performed using dox inducible shCTRL NB cells. (4) Fig.S4 shows the xenograft growth of shCTRL NB tumors and the murine survival of shCTRL tumor-bearing mice upon dox treatment. (5) Fig. S5 shows the polyploidy phenotypes in SY5Y, 293T, ARPE19 and HeLa cells. (6) Fig.S6 shows the induction of DNA damage response in shINCENP NB cells and the induction of apoptosis in both shINCENP NB cells and INCENP-depleted 293T, ARPE19 an HeLa cells. (7) Fig.S7 shows the cellular senescence phenotypes upon Aurora B inhibition or shINCENP mediated INCENP silencing in NB cells.