Supplementary Figure 5 from Neuropilin-1 Identifies a Subset of Bone Marrow Gr1− Monocytes That Can Induce Tumor Vessel Normalization and Inhibit Tumor Growth

PDF file - 3704K, The vessel normalization effect of NEMs is independent of tumor size. B16.F10 tumors were inoculated in mice, which were then injected at days 11 and 13 after tumor inoculation with PBS, Gr1+ cells and NEMs. Mice were sacrificed when tumors reached 1700 mm3 , irrespective of the day. A. Immunofluorescence analysis of the vasculature - representative images. Frozen sections were stained with antibodies against CD31 (green) and α-SMA (red).Nuclei are counterstained with DAPI. Scale bar: 100 μm. B. Quantitative evaluation of the α-SMA/CD31 ratio in vessels of tumors injected with PBS, Gr1+ cells or NEMs. Data are mean�s.e.m. **=P<0.01 vs. PBS.