Supplemental Figures 1 - 8 from Loss of Estrogen-Regulated microRNA Expression Increases HER2 Signaling and Is Prognostic of Poor Outcome in Luminal Breast Cancer

Supplementary Figure 1. Normalized NanoString counts for clusters differentially expressed in MCF7 vs. MCF7:2A cells. Supplementary Figure 2. ER binding is lower at binding sites found near differentially regulated miRNAs. Supplementary Figure 3. The expression of the individual miRNAs in the let-7c/miR-99a/miR-125b cluster is correlated in patient samples. Supplementary Figure 4. Kaplan-Meier plot examining the expression of let-7c, miR-99a, and miR-125b in the luminal B, Her2, basal, and normal-like PAM50 subsets. Supplementary Figure 5. Kaplan-Meier plots demonstrating that the luminal A low patient subset has survival similar to patients with luminal B tumors. Supplementary Figure 6. HER2 mRNA is slightly reduced in response to miRNA treatment. Supplementary Figure 7. Let-7c indirectly targets the HER2 UTR and regulates Dicer. Supplementary Figure 8. miR-125b expression is not negatively correlated with HER2 protein expression or activity in patients with breast cancer.