Supplementary Materials Figures and Tables from Myoferlin is a Key Regulator of EGFR Activity in Breast Cancer

PDF file, 2894K, Figure S1: Cell migration and invasion analysis following siRNA mediated silencing of myoferlin (MYOF) in MDA-MB231 cells. Figure S2: Myoferlin depletion in MDA-MB468 cells results in sustained EGFR phosphorylation upon EGF stimulation and impedes the EGF-induced cell migration. Figure S3: Analysis of time dependent pEGFR and EGFR expression patterns (48h post transfection with irrelevant siRNA) following EGF stimulation and chemical inhibition of the proteasome with MG132. Figure S4: Cellular adhesion assay on selected extracellular matrix proteins. Figure S5: Modulation of vimentin expression following myoferlin silencing in MDA-MB231 cells. Figure S6: Clathrin (CLH1) colocalizes with myoferlin and pEGFR during EGF mediated receptor activation and shows no modulation at the protein level following myoferlin silencing. Supplemental Tables Table S1: List of up-regulated breast tumor proteins obtained from the analysis of 3 non-tumoral adjacent and 3 tumoral specimens. Table S2: Average values (n=3) indicating the number of unique peptides, sequence coverage and score. Table S3: Glycosylated peptides observed for the proteins displayed in the Table 1.