Supplementary Figures 1-10 from Immune-Dependent and Independent Antitumor Activity of GM-CSF Aberrantly Expressed by Mouse and Human Colorectal Tumors

PDF file - 434K, (S1) GM-CSF expression and DNA methylation values at the GM-CSF promoter in human colon cancer cell lines; (S2) Association between LINE-1 and GM-CSF hypomethylation in human colon primary tumors; (S3) Characterization of mouse healthy colon (mHC), two murine colon cancer cell lines (MC38 and CT26), and the clones derived from Gm-CSf interference by stable transfection of those cell lines; (S4) Characterization of other independent clones derived from Gm-CSf interference by stable transfection of MC38 and CT26 cell lines and paracrine effects of Gm-CSf in vivo; (S5) Autocrine effects of Gm-CSf on tumor growth in vitro using an independent shRNA; (S6) Combination of autocrine and paracrine effects in vivo using an independent shRNA; (S7) Properties of wild-type MC38 cell line; (S8) Confocal representative images showing the expression of GM-CSF and its receptor in human colon primary tumors; (S9) Kaplan-Meier study of overall survival in 124 human paired colon samples; (S10) Published data of DNA methylation levels at the GM-CSF promoter and GM-CSF expression in human healthy primary samples.