Supplementary Figure 2 from NUP98 Fusion Oncoproteins Promote Aneuploidy by Attenuating the Mitotic Spindle Checkpoint

PDF - 1450K, Supplementary Figure 2. Proteasome inhibition rescues NUP98-fusions effects on Securin and Cyclin B degradation. Western blot analysis showing securin (SEC) and cyclin B (CCNB1) levels in HEK293 cells transfected with the indicated constructs after treatment with Nocodazole or Nocodazole and the proteasome inhibitor MG132 for 24 hours. An increase of CCNB1 levels can be appreciated in control cells following MG132 treatment as the result of efficient proteasome inactivation. Anti-betaactin antibody (betaACT) was used as a loading control.