Supplementary Figure S2 from TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation

Supplementary Figure S2 MiR-424 drives an intermediate EMT that does not repress epithelial programming. (A) Phase contrast images of SUM149PT cells stably expressing miR-424 and (B) miR-200c levels, by qPCR. MCF12A cells stably expressing miR-424 examined for (C) N-cadherin protein, (D) morphology and (E) miR-424 levels and (F) EMT markers, by qPCR. HMLE cells stably expressing miR-424 examined for (G) morphology, (H) miR-424 levels and (I) EMT markers, by qPCR, and (J) N-cadherin protein. SEM shown, n{greater than or equal to}3. *p<0.05, **p<0.01, ***p<0.001, two-tailed unpaired t-test. Bar-100 μm.