Figure S4 from Microenvironmental Cues Determine Tumor Cell Susceptibility to Neutrophil Cytotoxicity

Supplemental Figure S4. EMT increases TRPM2 expression and renders cells susceptible to neutrophil cytotoxicity. A. Representative images showing control and Snail overexpressing 4T1 cells. B. Snail expression in control and Snail overexpressing 4T1 cells. C. qPCR analysis of TRPM2, Vimentin (Vim.) and E-Cadherin (E-Cad.) expression in control and Snail overexpressing 4T1 cells. D. Extent of neutrophil cytotoxicity in co-culture with 4T1 control cells (Cont.) or 4T1 cells induced to undergo EMT by Snail overexpression (Snail). E. Representative images showing HMLE cells before (HMLE) and after prolonged treatment with 10 ng/ml TGFβ to induce EMT. F. qPCR analysis of TRPM2, Vimentin (Vim.) and E-Cadherin (E-Cad.) in control and TGFβâ^'treated HMLE cells. G. Extent of neutrophil cytotoxicity in co-culture with control (Cont.) or TGFβ-pretreated HMLE cells that have undergone EMT. H. Representative images showing control and Twist overexpressing HMLE cells. I. Twist expression in control and Twist overexpressing HMLE cells. J. qPCR analysis of TRPM2, Vimentin (Vim.) and E-Cadherin (E-Cad.) expression in control and Twist overexpressing HMLE cells. K. Extent of neutrophil cytotoxicity in co-culture with HMLE control cells (Cont.) or HMLE cells induced to undergo EMT by Twist overexpression (Twist). *p<0.05, **p<0.01.