Supplementary Figure 3 from Temporary Disruption of the Blood–Brain Barrier by Use of Ultrasound and Microbubbles: Safety and Efficacy Evaluation in Rhesus Macaques

PDF file - 130K, Figure S3: MRI and histological effects of sonication delivered at an exposure level that produced tissue damage in addition to BBB disruption. (A) T2*-weighted imaging after volumetric sonication (nine locations in a 3�3 grid) in the thalamus at 249 kPa. At three of the nine sonicated locations, severe hypointense spots were observed in T2*-weighted imaging, and they were persistent for the next six months (B) Microphotograph of an H&E stained section showing parenchymal damage (box) evident six months after sonication. Macrophage accumulation and scattered brownish granules (hemosiderin deposits, arrows) are also evident. (C-D) High-magnification view of hemosiderin deposits from another part of this sonicated region. Prussian blue staining (D) was used to verify that the light brown clusters evident in H&E were hemosiderin (B-C: H&E; D: Prussian blue; scale bars: B: 100 Mum; C-D: 20 Mum)