Supplementary Figures S1- S22. from Role of Chromatin Damage and Chromatin Trapping of FACT in Mediating the Anticancer Cytotoxicity of DNA-Binding Small-Molecule Drugs

Supplementary Figures S1-S4- Cytotoxicity data for four cell lines with all compounds used in the study. S5-S7 - C-trapping via western blotting with in three cell lines. S8-S9 - C-trapping via imaging on populations of cells with high and low magnifications. S10- Time dependence of c-trapping S11 - Comparison of drug accumulations in the nuclei of cells via autofluorescence. S12 - Effect of inhibition of MDR on cytotoxicity of the drugs. S13 - Comparison of the potency of c-trapping caused by different compounds in HCT116 cells S14. Quantitation of Histone H4 eviction. S15. Immunofluorescent staining of histone H3. S16. Comparison of patterns of c-trapping caused by high concentrations of the drugs. S17-S20 - Analyses of genome-wide distribution of FACT via ChIP-seq. S21. Assessment of DNA damaging properties of the compounds. S22. Assessment of correlations between different parameters measured. S