Supplementary Figures from Huwe1 Sustains Normal Ovarian Epithelial Cell Transformation and Tumor Growth through the Histone H1.3-<i>H19</i> Cascade

Supplementary Fig. 1 The genetic status of the KRAS, MYC and TP53 genes in ovarian cancer.Supplementary Fig. 2 CK8 staining in cultured mouse ovarian surface epithelial cells.Supplementary Fig. 3 Detection of Huwe1 knockout in MOSE cells.Supplementary Fig. 4 Huwe1 knockout inhibits cell proliferation.Supplementary Fig. 5 Deleting Huwe1 does not promote apoptosis in MOSE-Huwe1L/L-CreER cells.Supplementary Fig. 6 CK8 staining in tumors formed by MOSE-Huwe1L/L-CreER cells.Supplementary Fig. 7 RNAseq analysis of MOSE-Huwe1L/L-CreER and MOSE-Huwe1L/L-Cre cells.Supplementary Fig. 8 The knockdown efficiency of the H19 shRNA in MOSE-Huwe1L/L-CreER cells.Supplementary Fig. 9 The knockdown efficiency of the H1.3 shRNA in MOSE-Huwe1L/L-CreER and MOSE-Huwe1L/L-Cre cells. Supplementary Fig. 10 The knockdown efficiency of HUWE1 shRNA in SKOV-3 cells.Supplementary Fig. 11 Reduced HUWE1 expression inhibited proliferation in SKOV-3 cells.Supplementary Fig. 12 Staining for Ki67 and HUWE1 in tumors formed by SKOV-3/teton-shHUWE1 cells.Supplementary Fig. 13 HUWE1 binds to and ubiquitinates H1.3 in SKOV-3 cells.