Supplementary Figure S3 from Circulating Mitochondrial DNA Level, a Noninvasive Biomarker for the Early Detection of Gastric Cancer

Supplementary Figure S3 : Effect of smoking on the levels of mtDNA in peripheral leukocytes and serological IL-8 levels in NAG and GC patients, compared to healthy blood donors in cohort 1. A) Quantification of MtDNA levels by quantitative PCR as described in material and methods and B) Measure of IL-8 levels by ELISA. Cohort 1 included 47%, 48% and 56% of smokers in controls, NAG and GC groups respectively. Smokers are in black symbols and non-smokers in white symbols, circled symbols among GC cases in B) correspond to samples related to mtDNA inteval II. Each symbol represents a single patient, bars correspond to mean{plus minus}SEM. Each sample was tested in triplicate in three independent experiments. *p-value>0.05 ; **p-value<0.01.