Supplementary Figure 3 from NUP98 Fusion Oncoproteins Promote Aneuploidy by Attenuating the Mitotic Spindle Checkpoint

PDF - 2287K, Supplementary Figure 3. Mitotic index in transfected and in nocodazole arrested HEK293 cells. A, Mitotic index of control untransfected, nocodazole-arrested HEK293 and U937 cells. Cells were labelled with a specific anti-phosphoSer10 antibody and analysed by flow citometry. B, Immunoblot analysis of the expression of the indicated proteins in HEK293 cells transiently transfected with expression constructs for NUP98, NUP98-HOXD13, NUP98-LOC, NUP98-HHEX, treated with nocodazole, and harvested at the indicated time points. An arrowhead indicates the NUP98-HOXD13 band just below that of endogenous NUP98. CyclinB1 (CCNB1), Geminin (GMN) and securin (SEC) amounts were detected C, HEK293 cells, transiently transfected with expression constructs for the indicated proteins, were labelled with a specific anti-phosphoSer10 antibody and analysed by flow citometry.