Supplementary Figure 4 from NUP98 Fusion Oncoproteins Promote Aneuploidy by Attenuating the Mitotic Spindle Checkpoint

PDF - 1659K, Supplementary Figure 4. siRNA knock-down experiments. A, Immunoblots showing the effects of siRNAs on CDC20 or CDH1 protein expression. Anti-betaactin was used as a loading control. Graphs representing immunoblot quantitations are shown on the right side. B, Cell cycle analysis using propidium iodide staining and flow citometry on control siRNA-treated HEK293 cells (left side), and on siRNA-treated HEK293 cells expressing the NUP98-HOXD13 oncogene (right side).