Supplementary Table 1, Figure 1 - 6 from Soluble Carcinoembryonic Antigen Activates Endothelial Cells and Tumor Angiogenesis

PDF file - 482K, Supplemental Table 1 displays patients' characteristics of paraffin-embedded tumor tissue sections analyzed in Figure 6D; Supplemental Fig.1 demonstrates tumor-vascularization of CEA-mimotop immunized mice; Supplemental Fig.2 displays Matrigel-invasion as well as CaKi2-xenotransplant in vivo assays; Supplemental Fig.3 gives a description of the purity of CEA used in in vitro assays; Supplemental Fig4 reflects CEA-induced integrin activation and it's independency of VEGF; Supplemental Fig.5 gives a summary of CEAR expression as well as a potential CEA effect in different cell types; Supplemental Fig.6 displays consequences of CEACAM1 downregulation on CEA-induced intracellular signal transduction; a detailed description of the materials and methods used is given in the appendix of the Supplemental Information.