Supplementary Methods, Figures 1 - 6, Tables 1 - 4 from A Novel EGFR Isoform Confers Increased Invasiveness to Cancer Cells

PDF file - 734K, Figure S1. EGFRvA is widely expressed in various cancer cell lines and tissues. Figure S2. The upregulation of EGFRvA in glioma tissues compared with paired adjacent non-neoplastic brain tissues and a poor prognosis in patients with high-grade gliomas. Figure S3. EGFRvA promotes cell migration and invasion in vitro and in vivo. Figure S4. EGFRvA caused the activation of STAT3 and increased expression of HB-EGF. Figure S5. The positive feedback regulation between HB-EGF and p-STAT3 in EGFRvA-expressing cells. Figure S6. Cell viability and cell adhesion of U87MG EGFRvA cells treated with STAT3 inhibitor AG490, and siRNA-mediated knockdown of STAT3 in U87MG transfectants. Table S1. Clinical characteristics of patients with low or high expression of EGFR or EGFRvA in 52 glioma patients. Table S2. Higher expressed genes in U87MG EGFRvA cells versus U87MG EGFR cells. Table S3. Lower expressed genes in U87MG EGFRvA cells versus U87MG EGFR cells. Table S4. Primers used in this study.