Data Supplement from Validation and Structural Characterization of the LEDGF/p75–MLL Interface as a New Target for the Treatment of MLL-Dependent Leukemia

Supplementary Figures S1-S9. Published structure of the MLL-MENIN-LEDGF/p75 complex (S1); Characterization of the murine MLL-AF9 AML model (S2); Coomassie stained SDS-PAGE gels of recombinant proteins used in this study (S3); Identification of the MLL-LEDGF/p75 interaction surface (S4); Protein melting curves of LEDGF/p75 mutants (S5); Model of the MLL-MENIN-LEDGF/p75 ternary interface (S6); Representation of the buried surface area of IBD amino acid residues upon binding either MLL or HIV-1 integrase (S7); A MLL interaction deficient LEDGF/p75 mutant cannot rescue MLL-AF9 transformation upon LEDGF/p75 knockdown (S8); CP65 interferes with LEDGF/p75-MLL but not with MENIN-MLL complex formation in vitro (S9).