supplemental figures from Calcium-Sensing Receptor Promotes Breast Cancer by Stimulating Intracrine Actions of Parathyroid Hormone–Related Protein

Supplemental Figure 1. A&B) Whole mount preparations of mammary glands from 9-week-old virgin CaSR; Supplemental Figure 2. A) Representative immunoblot showing total cdk2 levels in BT474 cells {plus minus} NPS2143 or control, CaSRKD, and PTHrPKD BT474 cells at different calcium concentrations; Supplemental Figure 3. A) Representative TUNEL staining in control MDA-MB-231.1833 cells {plus minus} NPS2143, and CaSRKD and PTHrPKD MDA-MB-231.1833 cells at different calcium concentrations; Supplemental Figure 4. A) Representative immunoblot for AIF in cytoplasmic or nuclear extracts from cells cultured from control MMTV-PyMT tumors {plus minus} NPS2143 and exposed to 5mM extracellular calcium; Supplemental Figure 5. BrdU incorporation in CaSRKD (A) or PTHrPKD (B) MDA-MB-231.1833 cells {plus minus} 10nM or 100nM PTHrP.