Supplementary Materials and Methods, Supplementary Tables 1 through 5, and Supplementary Figures 1 through 10 from Definition of a Novel Feed-Forward Mechanism for Glycolysis-HIF1α Signaling in Hypoxic Tumors Highlights Aldolase A as a Therapeutic Target

Supplementary Materials and Methods Table S1: Data collection and refinement statistics native ALDOA and native ALDOA +TDZD8 Table S2: Data collection and refinement statistics: ALDOA-ND1 and ALDOA-ND1+TDZD-8. Table S3: Inhibition of HIF-1 activity by siRNA knockdown of glycolysis genes Table S4: Increase in glycolysis gene expression in hypoxia and the effect of siHIF-1 Table S5: Validation of screen results using alternate siRNAs Figure S1: Inhibition of HIF-1 activity and proliferation following knockdown of glycolysis genes Figure S2: Effect of knockdown of PGKs or ALDOA on HRE-luc activity on proliferation Figure S3: Effects of knockdown of ALDOA or PGK1 or PGK2 on ATP levels Figure S4: Effects of knockdown of ALDOA or PGK1 and PGK2 on HIF-1α protein levels Figure S5: Differential expression of ALDO or PGK Isoforms in tumor cells Figure S6: HIF-1 activity levels in MDA-MB-231 doxycycline inducible shALDOA clones sh8.8 and sh9.7. Figure S7: Stereo pairs of ALDOA monomer comparisons, shown as ribbon diagrams, with ligands shown as sticks Figure S8: TDZD-8 inhibition of HT-29, PANC1 and MIA PaCa-2 cancer cell proliferation, lactate formation, and HRE-luciferase reporter activity Figure S9: Effect of TDZD-8 on phospho-AMPK, phospho-p300 and ATP in PANC1 pancreatic cancer cells Figure S10: Effect of TDZD-8 treatment on levels of glycolytic intermediates and non-involved amino acids.