Supplementary Figures S1-S9 from ADAPT, a Novel Scaffold Protein-Based Probe for Radionuclide Imaging of Molecular Targets That Are Expressed in Disseminated Cancers

Analytical RP-HPLC analysis of purified DOTA-C-(HE)3-ADAPT6 ; Mass spectrum of purified DOTA-C-(HE)3-ADAPT6 ; Overlay of circular dichroism spectra from 250-195 nm for DOTA-C-(HE)3-ADAPT6 before (blue) and after (red) heating from 20-90{degree sign}C ; Normalized relative ellipticity of DOTA-C-(HE)3-ADAPT6 at 221 nm as a function of temperature ; SDS-PAGE analysis of 111In-DOTA-C-(HE)3-ADAPT6 ; Representative binding curve of the LigandTracer measurement of 111In-DOTA-C-(HE)3-ADAPT6 affinity using SKOV-3 cells ; InteractionMap of 111In-DOTA-C-(HE)3-ADAPT6 binding to SKOV-3 cells ; In vivo targeting specificity of 111In-DOTA-C-(HE)3-ADAPT6 (3 µg) in mice bearing SKOV-3 xenografts at 1 h p.i ; Biodistribution of 111In-DOTA-C-(HE)3-ADAPT6 in mice bearing SKOV-3 xenografts .