Figure S3 from <i>LINC00261</i> Is an Epigenetically Regulated Tumor Suppressor Essential for Activation of the DNA Damage Response

Figure S3. Characterization of stable H522-CMV-LINC00261 cell lines. A. qRT-PCR levels of LINC00261 expression in H522 cells transfected with ectopic CMV-LINC00261 and CMV-NEO backbone as the control vector. Three stable cells lines of each construct were generated. B. IGV image of LINC00261 locus (hg19). Blue = BigWig tracks of primary AEC RNA expression. Reads are binned to 30bp windows. Purple = BigWig tracks of H522 cell lines stably selected with either CMV-LINC00261 ectopic expression or CMV-NEO control vector.C. Quantification of in vitro cell migration shown in Figure 2B using T-Scratch Software. Paired t-test was used for statistical significance. D. Gating images of FACS analysis from Figure 2C. Live cells were selected by exclusion of cell debris. Representative images shown.