Figure S5 from Erbin Suppresses KSR1-Mediated RAS/RAF Signaling and Tumorigenesis in Colorectal Cancer

Figure S5. Loss of Erbin deletion promotes tumorigenesis and the expression of genes associated with cancer stem cells. (A) Histology analysis of intestine adenomas in age-matched Apc/WT and Apc/Het mice (at 4.5 months) as well as Apc/WT mice at 6 months. Scale bar, 200μm. Note that the tumor burden in the Apc/Het mouse at 4.5 month is similar as that Apc/WT mice at 6 month. (B) Tumor organoids derived from three cohorts of mice (Apc/WT, Apc/Het and Apc/KO) were grown in 3D culture for 72 hours, collected, and analyzed using qRT-PCR for the expression of genes associated with cancer stem cells (Lgr5 and Axin2), cell proliferation (Ccnd1, Ki67) and intestinal cell differentiation (Fabp2). Data represents mean {plus minus} SEM (n = 4, * p<0.05 and # p<0.0001).