Supplementary Figure 1 from Identification of an Aurora Kinase Inhibitor Specific for the Aurora B Isoform

PDF file - 107K, HOI-07 inhibits Akt1 and Akt2 activities in vitro but not in cells. A and B, HOI-07 inhibits in vitro Akt1 (A) and Akt2 (B) kinase activities in a concentration-dependent manner. Akt1 and Akt2 kinase assays were performed using the liquid scintillation method. C and D, Western blot analysis of the expression of Akt downstream signaling molecules in A549 (C) and NCI-H520 (D) cancer cells. No obvious inhibitory activity on the Akt downstream pathway was observed after treatment HOI-07 for 2 h