Supplemental Figures and Tables from Notch4 Signaling Induces a Mesenchymal–Epithelial–like Transition in Melanoma Cells to Suppress Malignant Behaviors

SF1: Phenotypic and functional changes of 451Lu, WM9 and WM164 following N4ICD overexpression SF2: Immunoblots and immunohistochemistry of EMT markers and Notch4 SF3: Details of CSL binding to Snail2 and Twist1 promoter regions SF4: Immunoblots of overexpression and silencing experiments confirming the Notch4-Hey1/2-Snail2/Twist1 axis (SF4) SF5: Immunoblots of pLNCX-N4ICD and siHey1/2 transduced cells and details of Snail2 and Twist1 promoter regions ST1: Plasmids and siRNAs ST2: Antibodies ST3: Primers for qPCR and ChIP ST4: Sequence of EMSA fragments ST5: Sequence of luciferase fragments ST6: Fold regulation of EMT markers determined by PCR-array ST7: Details of immunohistochemical analyses of melanoma samples Supplementary references