Supplementary Figure 2 from Critical Role of STAT3 in IL-6–Mediated Drug Resistance in Human Neuroblastoma

Supplementary Figure 2 - PDF file 179K, A, SK-N-SH cells were transfected with a STAT3 siRNA nucleotide or a scrambled sequence as described in Materials and Methods. After 72 hours, cells were treated with IL-6 (10 ng/mL) and sIL-6R (25 ng/mL) for 30 minutes and cell lysates were examined for expression of pSTAT3 and STAT3 by Western blot. The data are representative of 3 separate experiments. B, In parallel experiments, siRNA transfected CHLA-255 cells were treated with IL-6 and sIL-6R for 24 hours for Annexin V expression by flow cytometry. The data represent the mean (�SD) percentage of Annexin V positive cells of the control (treated with etoposide, 0.25 mg/mL) from three independent experiments. C, SK-N-SH cells were treated with IL-6 (10 ng/ml) at indicated time points and total cell lysates (20 �g) were obtained and examined for the expression of anti-apoptotic proteins by Western blot. Actin was used as loading control. The data are representative of 2 separate experiments showing similar results