Supplementary Materials and Methods from Human Helicase RECQL4 Drives Cisplatin Resistance in Gastric Cancer by Activating an AKT–YB1–MDR1 Signaling Pathway

Dongliang Mo,Hongbo Fang,Kaifeng Niu,Jing Liu, Meng Wu, Shiyou Li, Tienian Zhu,Mohammed A. Aleskandarany,Arvind Arora, Dileep N. Lobo,Srinivasan Madhusudan,Adayabalam S. Balajee,Zhenfen Chi,Yongliang Zhao

crossref（2023）

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Abstract

1. Construction of Tissue Microarray (TMA). 2. Chemicals. 3. Real-time PCR. 4. Dual-Luciferase assay for MVP. 5. In vitro pull-down assay. 6. Co-immunoprecipitation. 7. Protein expression and purification. 8. Chromatin immunoprecipitation analysis 9. Electrophoretic mobility shift assays (EMSAs). 10.PCR-SSCP protocol.

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