Supplementary Figures and Table from Hypercholesterolemia Increases Colorectal Cancer Incidence by Reducing Production of NKT and γδ T Cells from Hematopoietic Stem Cells

This file contains 9 supplemental figures and one supplemental table in which we show that WT recipient mice reconstituted with HSCs from hypercholesterolemic ApoE-/- mice have normal serum cholesterol levels and white blood cell counts (S1); Hypercholesterolemia reduces the frequency and alters specific subsets and maturation of NKT and gammadelta T cells in the thymus (S2); Hypercholesterolemia-induced oxidant stress in HSCs strongly correlates with the incidence of AOM-induced colorectal neoplasia (S3); The frequency of T cell intermediate populations in thymus and cellular components related to cancer immunosurveillance in peripheral blood and colon of WT and Tet-/- mice (S4); The expression of Tet1 determines the differentiation of HSCs towards NKT and gammadelta T cells in vitro (S5); The expression of miRNA in HSCs from hypercholesterolemic mice (S6); That In HSCs, Tet1 overexpression reduces the expression of the key regulatory genes in their differentiation of NKT and gammadeltaT cells (S7); That Tet1 regulates H3K4me3 modification of the key regulatory genes in the differentiation of HSCs towards NKT and gammadeltaT cells in vitro (S8); That oxLDL impairs the differentiation of human HSCs towards NKT and gammadelta T cells in vitro (S9); and the genes related to the differentiation of NKT and gammadelta T cells (Supplemental Table 1).