Data Supplement from AR-Regulated TWEAK-FN14 Pathway Promotes Prostate Cancer Bone Metastasis

Supplemental Figure 3. (A) Western blot analysis of the DU145/RasB1IκBαSR cell line, with or without prior doxycycline induction for 2 days, harvested at various time points (min) after TWEAK treatment (50ng/ml) and assayed for the proteins indicated. (B) In vitro growth of the DU145/RasB1IκBαSR cell line with and without prior doxycycline induction. *p<0.05. (C) Average body weight of mice bearing DU145/RasB1IκBαSR tumors in mice fed with doxycycline on day 0 (n=9) or day 14(n=9). **p<0.01 vs control (n=8). (D) Western blot analysis of Fn14, IKKB and NIK levels in DU145/RasB1Fn14KD cell lines rescued by re-expression of Fn14, IKKBS177E S188E , and NIK∆T3.