Supplementary Figure 3 from Clusterin Mediates TGF-β–Induced Epithelial–Mesenchymal Transition and Metastasis via Twist1 in Prostate Cancer Cells

Masaki Shiota,Anousheh Zardan,Ario Takeuchi,Masafumi Kumano,Eliana Beraldi,Seiji Naito,Amina Zoubeidi,Martin E. Gleave

crossref（2023）

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PDF file - 48K, PC-3 cells were transfected with 40 nmol/L of the indicated siRNA. At 48 h after transfection, the media was changed to serum-free media +/- 0.1 ng/mL TGF-beta, and incubated for 24 h. Whole-cell extracts were analyzed by SDS-PAGE and western blot analysis with specified antibodies

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